| Abstract Detail
Conference Wide Chae, Young [1], Park, Jong-sug [2], Kim, Ki-Taek [3], Cho, Myeong-Cheoul [1], Cho, Yong-Seop [4], Pae, Do-Ham [1], Mok, Il-Gin [5], Ko, Kwan-Dal [1], Oh, Dae-Geun [6], Yi, Bu-Young [7]. Screening of differentially expressed genes related to anthracnose resistance in progenies derived from interspecific hybrid between Capsicum baccatum var. pendulum and C. annuum. THIS study was undertaken to characterize the mechanism of resistant gene(s) derived from interspecific hybrid lines between C. baccatum var. pendulum and C. annuum in the molecular level. The specifically or differentially expressed genes in the resistant or susceptible lines after artificial inoculation were screened. Differentially expressed genes (DEG) identified by a combination of dT-ACP2 (reverse primer) and 120 arbitrary ACP primers (forward primer) were detected by ACP-based PCR method using the GeneFishingTM DEG kits. Twenty-three differentially expressed cDNA bands were appeared on the anthracnose resistant lines whereas no band was developed in susceptible lines. By sequence analysis of the differentially expressed cDNAs, it was found that 5 DEGs were matched to sequences in NCBI GenBank databases with high homology. GP26 sequence was matched to the S-adenosyl methionine synthaseI with 72% homology, GP71 to Snakin2 with 71% homology, GP80 to DDI1 with 66% homology, GP92 to tryptophan synthase 2 beta chain sequence with 75% homology, and GP108 to pepper mild mottle virus sequence with 76% respectively. Nine of the other DEGs were matched with unknown functions hypothetical proteins with highly significant homology. In summary of the DEG results, the hypersensitive reaction of the interspecific hybrid lines against anthracnose might be due to a series of molecular biological mechanism responding to the invasion and multiplication of the anthracnose fungi. In this study it was found that the synthesis of antimicrobial components, amino acids and proteins, repairing mechanism of damaged DNA, and adaptation to stress followed by the invasion of fungi were all enhanced. Finally, it would be needed to analyze all the detected DEGs to fully understand mechanisms of the resistant and/or susceptible gene(s) responsible for the anthracnose resistant in pepper.
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Related Links:
1 - National Horticultural Research Institute, Vegetable Research Division, 475, Imok-dong, Jangan-gu, RDA, Suwon, 440-706, Korea
2 - National Institute of Agricultural Biotechnology, Research Planning & Information Division, 224 Suinro, Gweonseon-gu, RDA,, Suwon, Gyeonggi-do, 441-707, Rep. of Korea
3 - National Horticultural Research Institute, Horticulture Biotechnology Division, 540-41 Tap-dong, Suwon, 441-440, Rep. of Korea
4 - National Horticultural Research Institute, Protected Horticulture Experiment Station, 475, Imok-dong, Jangan-gu, RDA, Suwon, 440-706, Rep. of Korea
5 - National Institute of Agricultural Biotechnology, 475, Imok-dong, Jangan-gu, RDA, Suwon, 440-706, Rep. of Korea
6 - National Horticultural Research Institute, Horticulture Biotechnology Division, 540-41 Tap-dong,, Suwon, 441-707, r
7 - University of Seoul, Department of Environmental Horticulture, Seoul, 130-743, Rep. of Korea
Keywords:
Capsicum annuum
capsicum baccatum
Iinterspecific hybrid?>
anthracnose
hot pepper
differentially expressed genes.
Session: Poster-91
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:126 |
