| Abstract Detail
Plant Protection Liu, Zhenyu [1], Halterman, Dennis [2]. Proteomic Analysis of Potato Late Blight Resistance Mediated by the RB Resistance Gene. LATE blight, caused by the oomycete pathogen, Phytophthora infestans, is a devastating disease of potatoes and tomatoes. A gene RB, cloned from the Mexican diploid potato species Solanum bulbocastanum, confers robust resistance to potato late blight. RB encodes a protein belonging to the CC-NBS-LRR (coiled coil-nucleotide binding site-leu-rich repeat) class of resistance proteins. It is likely involved in perception of Phytophthora and signaling to elicit resistance within the plant cell cytoplasm. We are using proteomics to understand the disease resistance mechanism mediated by RB. Complex protein mixtures are first separated by two-dimensional electrophoresis (2DE) according to charge and size (molecular weight). Selected proteins resolved by 2DE are then identified by mass spectrometry (MS). In these experiments, we compared proteins isolated from a late blight resistant transgenic potato plant SP951, containing a single copy of RB, with those from the susceptible control Katadhin (Solanum tuberosum cv. Katadhin) at 0, 24, and 72h post-inoculation using 2DE followed by MS.
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1 - University of Wisconsin-Madison, Department of Plant Pathology, 1630 Linden Drive, Madison, WI, 53706, USA
2 - USDA/ARS University of Wisconsin-Madison, Plant Pathology, 1630 Linden Drive, Madison, WI, 53706, USA
Keywords:
Potato
proteomics
Disease resistance
Late Blight.
Session: Poster-3
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:91 |
