| Abstract Detail
Conference Wide Schafleitner, Roland [1], Gutierrez Rosales, Raymundo [2], Gaudin, Amelie [2], Alvarado Aliaga, Carlos [2], Nomberto, Giannina [2], Bonierbale, Merideth [3]. Capturing candidate drought tolerance traits in two Native Andean potato clones by transcription profiling of field grown plants under water stress. GENE expression profiling, combined with agronomical, physiological and metabolite analysis was applied to investigate the drought responses of two moderately water stress tolerant Solanum tuberosum ssp. andigena clones, SA2563 and Sullu, in order to identify characteristics that contribute to drought tolerance. Drought-induced root growth was observed in SA2563, and in Sullu, leaf mass was strongly reduced, while osmolytes such as sugars and proline accumulated to high concentrations in leaves, giving a first indication of differences in the drought response in these two clones. Gene expression analysis using the TIGR 10k microarray revealed in total 1587 differentially expressed genes in leaves of drought-challenged and control plants of both clones at two different time points. 170 genes were up-regulated in both clones and encoded proteins functioning in transcription control, cellular regulation, transport, carbon-, amino acid-, lipid- and secondary metabolism, stabilization of membranes and proteins or previously implicated in stress response. Additionally to these commonly up-regulated genes, each clone expressed a specific gene set in response to drought. Beside hormone- and polyamine metabolism related genes, the clone-dependent activation comprised mostly genes acting in the response pathways mentioned above, but the number of up-regulated genes per pathway differed and different pathways were activated to various extents in the two clones under water stress. Down-regulated genes encoded mostly proteins implicated in photosynthesis and growth. The number of down-regulated genes differed significantly between the clones, with SA2563 showing a greater number of drought-repressed genes than Sullu. These data allowed us to establish a list of candidate drought tolerance traits present in these two clones. Further investigation of variations in these traits in potato populations and germplasm will reveal the relative importance of the candidate traits in drought tolerance and will allow selecting genes for water stress tolerance breeding.
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1 - International Potato Center (CIP), Germplasm and Crop Improvement Division, Avenida La Molina 1895, Apartado 1558, La Molina, Lima, 12, Peru
2 -
3 - International Potato Center (CIP), Crop Improvement and Germplasm Enhancement, Av. La Molina 1895, Lima 12, Apartado 1558, Peru
Keywords:
none specified
Session: Poster-171
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:525 |
