| Abstract Detail
Potato Genomics - Afternoon Aversano, Riccardo [1], Di Dato, Francesco [1], Frusciante, Luigi [2], Carputo, Domenico [1]. Studying molecular changes in synthetic polyploids of Solanum by AFLP analysis. ALTHOUGH the effect of polyploidization has been well studied in disomic polyploids, little is known about polyploids with polysomic inheritance, as S. tuberosum (2n=4x=48). Understanding the effect of polyploidization at whole genome level would be very important for the studying the polysomic polyploid formation process, that has important implications not only from the full exploitation of polyploidy for breeding purposes, but also for evolution and genetic studies. Towards this goal, we used two diploid (2n=2x=24) Solanum species, S. bulbocastanum (blb) and S. cardiophyllum (cph), and their in vitro-regenerated genotypes, all characterized for Phytophthora infestans resistance. We have performed AFLP analysis on 28in vitro regenerated 4x (14 from blb, 14 from cph), 10 regenerated 2x (5 from blb, 5 from cph), and on the parental genotypes they derived from. AFLPs allowed the detection of polymorphisms of interest between the regenerants with different ploidy levels. Bands present in 2x clones were absent in their 4x derivatives. By contrast, AFLP bands lacking in 2x genotypes were ex-novo detected in their 4x derivatives. Although the synthetic tetraploids maintained resistance to P. infestans, they were significantly distant from 2x parents. Indeed the similarity index between the diploid parents and synthetic tetraploids was much lower than that between 2x parents and 2x regenerants. This suggests a strong effect of polyploidization on the genetic variability found.
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1 - University of Naples Federico II, Department of Soil, Plant and environmental Sciences, Via Università 100, Portici (NA), Italy, 80055
2 - University of Naples “Federico II”, Dept Soil, Plant and Environmental Sciences, via università 133, Portici, 80055, Italy
Keywords:
Phytophthora infestans
wild potato species
Molecular markers.
Session: SAT09-13
Location: Hall of Ideas Room I/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 5:40 PM
Abstract ID:508 |
