| Abstract Detail
Conference Wide Handa, Avtar [1], Srivastava, Alka [2], Datsenka, Tatsiana [2], McIntyre, Lauren [3]. Transcriptional regulation during tomato fruit development: Identification of novel genes involved in fruit maturation and ripening. A tomato (Solanum lycopersicum.) cDNA macroarray containing in excess of 1000 unique fruit expressed cDNAs was developed and used to understand transcriptional circuitries during fruit development including ripening. We identified a total of 364 expressed sequence tags (ESTs) of which 117 were considered statistically significant at p≤0.0000811 and the remaining 247 were considered as interesting (p≤0.05 but greater than 0.0000811). Forty four percent of these encompass ESTs encoding unknown proteins or ESTs that show no match in the databases (novel). Approximately 24% of the differentially expressed ESTs are involved in metabolism and protein biosynthesis/degradation related functions with over 100 ESTs encoding for genes involved in defense, signaling, transcription, transport and cell structure and cell wall related functions. Expression profiling of these ESTs indicates that early fruit maturation and onset of ripening is perceived as a stress phenomenon since many defense and stress related genes are up-regulated at these stages. The mature green stage of the fruit appears to be a transcriptionally quiescent stage with a large number of differentially expressed ESTs (65) showing more than two fold decline in their transcript levels. A coordinated increase in expression profiles of many novel ESTs and ESTs of unknown functions along with the ESTs encoding for genes with documented functions in fruit development and ripening indicates a possible role for these ESTs in the development of fruit. Taken together, our results provide a basis for identifying many additional genes that play roles in fruit maturation and ripening.
Log in to add this item to your schedule
1 - Purdue University, Department of Horticulture and Landscape Architecture, 625 Agriculture Mall Drive, West Lafayette, IN, 47906, USA
2 - Purdue University, Horticulture and Landscape Architecture, West Lafayette, IN, 47907, USA
3 - Purdue University, Computational genomics, Department of Agronomy, Lilly Hall of Life Sciences, 915 W. State Street, West Lafayette, IN, 47907, USA
Keywords:
tomato fruit
Solanum lycopersicum
gene expression.
Session: Poster-168
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:492 |
