Unable to connect to database - 08:56:33 Unable to connect to database - 08:56:33 SQL Statement is null or not a SELECT - 08:56:33 SQL Statement is null or not a DELETE - 08:56:33 Solanaceae 2006 - Abstract Search
Unable to connect to database - 08:56:33 Unable to connect to database - 08:56:33 SQL Statement is null or not a SELECT - 08:56:33

Abstract Detail


Breeding and Genetics

Sadder, Monther, T. [1], Al-Shareef, Rami, M. [1], Haitham, Hamdan [2].

Assessment of genetic diversity among Jordanian eggplant (Solanum melongena L.) landraces using random amplified polymorphic DNA (RAPD).

GENETIC variation among ten Jordanian eggplant landraces (E4, E12, E17, E18, E21, E22, E23, E24, E27, E29) in addition to three cultivars (hybrid Shanta, Shanta, and Samar) were assessed. No variation was found using non-transcribed spacer of the 5S rDNA. In addition, RAPD analysis of the genomic DNA was done using 9 primers after screening 38 different primers, which gave a total of 85 bands (81 were polymorphic). E22 showed the highest amplified fragments (72), while E12 gave 8 fragments (the average was 42 fragments per genotype). To assess genotype similarities, a total of 1053 data entries were utilized (523 present and 530 absent bands) using Jaccard’s similarity coefficient, and a UPGMA dendrogram was constructed from similarity values. The maximum similarity recorded was 80.5% between genotypes E22 and E23, while the most distant genotypes (E12 and Ec2) were having 4.8 % similarity. E12 did not cluster at all, while the remaining clustered into two major clades; clade I with 10 genotypes (E22, E23, E17, E21, E18, Ec1, E27, E24, Ec2, E4) and clade II with two genotypes (E29, Ec5). Polymorphic index content was calculated for each marker, with an average value of 0.391. A 23% of markers showed high values (0.497), while 9.5% showed low values (0.142). According to D value, primers C02 and RAPD02 could differentiated between all genotypes (D=1), however, other primers need to be combined to discriminate all genotypes. The best combination between other primers was containing C01, C15 or RAPD03. landraces were evaluated and characterized under Jordan Valley conditions. Considerable differences in plant growth habit, yield, and fruit charachteristics were observed among the genotypes. These germoplasm can be used by breeders in breeding programs according to their objectives. Furthermore, higher yield in E18 and E29 is useful for further eggplant salt tolerance studies.


Log in to add this item to your schedule

1 - University of Jordan, Horticulture and Crop Science, Faculty of Agriculture, University of Jordan, Amman, 11942, Jordan
2 - NCARTT, National Center for Agricultural Research and Technology Transfer, P.O. Box 639, Baq’a, 19381, Jordan

Keywords:
Figerprinting
eggplant
landraces.


Session: Poster-35
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:485


Copyright © 2000-2006, Botanical Society of America. All rights