| Abstract Detail
Plant Protection Sadder, Monther, T. [2], Al-Banna, Luma [3], Khyami-Horani, Hala [1], Abdeen, Amany [2], Abu Al-ragheb, Isa�af [3], Abu-Dhaim, Eman [3]. Jordanian Bacillus thuringiensis isolates against root-knot nematodes (Meloidogyne sp.) in tomato. SEVEN Jodrainan Bacillus thuringiensis (Bt) isolates, which have been analyzed for toxicity against important pests, were also differentiated through serotyping. In this study, they were analyzed at the molecular level using random amplified polymorphic DNA markers. Five more international strains were incorporated in the analysis. The used DNA markers showed high polymorphism among the tested isolates. However, the data did not align completely with earlier serotyping for most isolates. Therefore, it is recommended to engage several analyses (e.g. biochemical and molecular) when classifying newly surveyed Bt isolates in the world. Some of these local and reference strains were tested against root knot nematodes Meloidogyne incognita and M. javanica. Exposing freed eggs or eggs within eggmasses to 106 viable spores/ml of each local strain resulted in either complete inhibition or few J2s hatching of both nematode species. Lower concentrations (105 viable spores /ml) were less effective in reducing egg hatching. Depending on the local Bt strains, 100% mortality of J2 of both nematodes occurred either after 2, 4 or 6 days of exposure to the higher spore concentration. Lower concentration of Bt strains was less effective but with a significant increase of mortality of J2s of both nematodes. Furthermore, reduction in tomato root galling was 51-59% when M. javanica eggs or second stage juveniles (J2) were used as inoculum, respectively. Single (14days) and double (14 and 7 days) applications of Btj before plantation, both resulted in a significant reduction in root galling.
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1 - University of Jordan, Bio. Sciences, Faculty of Sciences, University of Jordan, Amman, 11942, Jordan
2 - University of Jordan, Horticulture and Crop Science, Faculty of Agriculture, University of Jordan, Amman, 11942, Jordan
3 - University of Jordan, Plant Protection, Faculty of Agriculture, University of Jordan, Amman, 11942, Jordan
Keywords:
bacillus thuringiensis
nematodes
tomato.
Session: Poster-15
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:484 |
