| Abstract Detail
Breeding and Genetics Sadder, Monther, T. [1], Al Abdallat, Ayed [1], Suwwan, Mohammad [1], Abdeen, Amany [1]. Functional genomics of tomato beta-glycosidases (beta-glucosidases [EC 3.2.1.21] and beta-galactosidases [EC 3.2.1.23]): phylogenetic analysis and cloning. O-GLYCOSIDE hydrolases (EC 3.2.1.-) are enzymes that catalyze the cleavage of chemical bonds between two or more carbohydrates or between a carbohydrate and a non-carbohydrate moiety. Two major families are of special focus. Family 1 includes beta-glucosidases (beta-D-glucoside glucohydrolase, EC 3.2.1.21) and thioglucoside glucohydrolases (myrosinases; EC 3.2.3.1), which function in higher plants in chemical defense against herbivores and pathogens, lignin biosynthesis, and plant growth and development. Family 35 contains the beta-galactosidases (EC 3.2.1.23), which play key roles in fruit ripening, flower senescence, mobilization of carbohydrate reserves, and galactolipid turnover. Arabidopsis contains approximately putative 75 members of both families. However, in tomatoes, only few were cloned and characterized. Several plant beta-glucosidases were aligned with ClustalW to design general degenerated primers. However, cloned fragments did not match except centromeric regions. One more trail was done using the database of SOL Genomics Network, where eight putative tomato beta-glucosidases were constructed using ETS contigs. The sequences were exposed to six-way reading frames to get the most suitable ORF. However, the protein sequences did not align well using ClustalW neither among each nor with other beta-glucosidases from other plant species. RT-PCR was conducted in total RNA from tomato leaves to amplify those eight genes. The cDNAs were cloned and sequences from both sides. Only one known tomato beta-glucosidase, which was cloned earlier from fruits, was correct. However, the remaining clones were not similar to any of the original sequences.
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1 - University of Jordan, Horticulture and Crop Science, Faculty of Agriculture, University of Jordan, Amman, 11942, Jordan
Keywords:
beta-glycosidases
tomato.
Session: Poster-50
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:483 |
