| Abstract Detail
Breeding and Genetics Watanabe, Kazuo [1], Tahara, Yusaku [1], Kikuchi, Akira [1], Yamanaka, Shinsuke [2]. Herbicide tolerances with mammalian cytochrome P450 genes on potato varieties. MAMMALIAN cytochrome P450s (CYPs) detoxify xenobiotics such as herbicides by its enzymatic catalytic function. Animal CYPs have a broad range of substrate reactions, and the effect of single CYPs was tested on a Japanese potato variety by Inui et al. In general mammalian CYP genes support tolerances to various commercial herbicides with the detoxyfication function in plants. CYP1A1, CYP2B6 and CYP2C19 genes derived from human liver microsomes were constructed together with CaMV35S promoter, and Agrobacterium-mediated transformation was conducted to Desiree. After ordinary molecular biology confirmation of the presence of transgenes by Southern hybridization and expression by RT-PCR, we have examined 439 independent lines on their tolerance to different chemical properties of herbicides. Atrazine, Chlortoluron and Methabenzchiazuron were used respectively to the in vitro plantlets of the transgenic lines. A total of 40 lines provisionally indicated tolerance to the three herbicides. And potted plants of the all forty transgenic lines indicated prominent tolerance to Chlortoluron compared with their non-transgenic cultivars. With Atrazine and Methabenzchiazuron, some of the potted plants of transgenic lines indicated significantly higher tolerance than their control counterparts. The tolerance was supported by the metabolism of the chemical property of the herbicides by the function of CYPs. We have also generated transgenic potato lines using four different cultivars, Atlantic, Dejima, Pito and Yucon Gold to seek for general utility possibility of CYP genes for herbicide tolerance in potatoes.
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1 - University of Tsukuba, Gene Research Center, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8572, Japan
2 - National Institute of Agrobiological Resources, Genebank, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan
Keywords:
herbicide
transgenic
tolerance
expression profiling
xenobiotics.
Session: PAA07a-6
Location: Hall of Ideas Room G/Monona Terrace
Date: Monday, July 24th, 2006
Time: 2:45 PM
Abstract ID:48 |
