| Abstract Detail
Conference Wide Rolland, M. [1], Tribodet, M. [2], Croizat, F. [2], Lefeuvre, P. [2], Balme-Sinibaldi, V. [1], Bonnel, E. [3], Kerlan, C. [2], Jacquot, E. [2]. Characterisation using innovative tools of potato tuber necrotic Potato virus Y (PVY) isolates. POTATO virus Y (PVY) is the type-member of the genus Potyvirus (family Potyviridae). Based on the different symptoms induced on indicator host plants (e.g. Nicotiana tabacum cv Xanthi, Solanum tuberosum ssp tuberosum), PVY potato isolates have been classified into PVYO, PVYC and PVYN groups. Moreover, due to their biological properties and molecular characteristics, some PVYN isolates have been classified as members of the PVYN-W or PVYNTN variants. As an alternative to biological assays, several serological and molecular detection tools were progressively developed according to the upgrading knowledge on PVY. Most of these tools (separately or in combination) are able to identify the PVY group and/or variant for almost all PVY potato isolates tested. However, they are based on the use of neutral markers (antigenic determinants, sequence data, recombination sites or restriction enzyme cleavage sites). In consequences, these tools could fail in characterisation of PVY isolates that possess genomic determinants of biological properties unlinked to these neutral markers. The recent identification of single nucleotides involved in the induction of the tobacco leaf necrosis symptom has permitted to set up innovative detection/characterisation tools. The latter are based both marker(s) linked to the major biological property used to establish the taxonomy of PVY and on the use of different technologies including either one-step gel-free qualitative and quantitative “real-time” RT-PCR or original fluorescent-based strategies. These new assays specifically detect and quantify PVYO and PVYN isolates. Furthermore, the characteristics of the developed procedures for PVY detection make it possible the specific characterization of PVY groups and variants in a single reaction. The specificity and sensitivity of these new detection techniques were tested using a wide range of PVY isolates and assessed using both pure and mixed suspensions containing PVYO, PVYN, PVYN-W and/or PVYNTN particles.
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1 - Fédération Nationale des Producteurs de Plants de Pomme de Terre, 9 rue d´Athènes, Paris, 75009, France
2 - INRA/Agrocampus-Rennes, UMR Biologie des Organismes et des Populations appliquée à la Protection des Plantes, Domaine de la Motte, Le Rheu, 35653, France
3 - Groupement national interprofessionnel des semences et plants, 44 rue du louvre, Paris, 75001, France
Keywords:
none specified
Session: Poster-164
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:450 |
