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Abstract Detail


Breeding and Genetics

Herrera, Maria del Rosario [1], Jorge, Nuñez [2], Guzman, Frank [1], Ghislain, Marc [2], Spooner, David [3].

A new potato genetic identity kit of 24 SSR markers for high throughput fingerprinting of large collections.

A large fingerprinting project of potato landraces and breeders material, about 800 genotypes, was developed with 56 SSR markers which resulted in revisiting the proposed potato genetic identity kit made of 18 SSR makers (Ghislain et al., 2004. TAG 108: 881-890). The new SSR markers were mapped on the potato genetic linkage map. All informative SSR markers (46) on a collection of 531 landraces were characterized by their polymorphic information content (PIC). These results allowed us to select a new PGI kit based on the following criteria: 2 SSR markers (>10 cM linkage distance) per chromosome, high PIC value, and high quality of amplicons. The new proposed PGI kit consists of 11 SSR markers from the first kit and 13 new SSR markers. The comparison of similarity matrix (Ntsys) of the 531 landraces obtained with the 24 SSR of the new kit and with 32 SSR markers resulted with a high correlation (r = 0.97) by Mantel test. The new PGI kit is able to discriminate 96.8% of the 531 landraces whereas the 46 SSR markers 98.9%. Reference DNA sample for the 24 SSR PGI kit have been identified in order to compare and identify unambiguously SSR alleles using M13-tailed SSR and the Licor 4300 electrophoresis system. This new PGI kit will be of particular utility to compare fingerprints of potato cultivars among breeders and gene banks worldwide.


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1 - International Potato Center, Applied Biotechnology Laboratory, PO Box 1558, Lima, L-12, Peru
2 - International Potato Center (CIP), Crop Improvement and Germplasm Enhancement, Av. La Molina 1895, Lima 12, Apartado 1558, Peru
3 - University of Wisconsin, Department of Horticulture, 1575 Linden Drive, Madison, WI, 53706, USA

Keywords:
Fingerprinting
Potato
ssr.


Session: Poster-24
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:438


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