| Abstract Detail
Application of FISH in Support of Sequencing Plant Genomes - Afternoon Wang, Ying [1], Cheng, Zhukuan [2]. Application of FISH in support of sequencing plant genomes. FLUORESCENT in situ hybridization (FISH) has been widely used for cytogenetic studies, especially high resolution chromosome analysis in support of sequencing the genomes. First, genomes can be characterized with FISH, including estimating the genome size, the genome composition, and chromosome structure. Secondly, FISH of large insert clones, BACs and PACs, or repetitive DNA fragments can greatly facilitate the construction of physical map and identifying the clones for BAC-by-BAC whole genome sequencing. FISH has been successfully guiding the whole genome sequencing of model dicot species, tomato (Solanum lycopersicum). Eleven sequenced BACs are annotated and localized via FISH to tomato pachytene chromosomes – providing the first global insights into the compositional differences of euchromatin and pericentromeric heterochromatin in tomato. BACs with ambiguous mapping position on the genetic linkage maps are localized on tomato chromosomes with one or more reference BACs, suggesting the accurate physical locations for identifying the minimum tilling path and assembling the BAC sequences.
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1 - Wuhan Botanical Garden, Chinese Academy of Sciences, Moshan, Wuchang,, Wuhan, Hubei, 430074, China
2 - Institute of Genetics and Developmental Biology, Chinese Academy of Sc, Beijing, Beijing, 100101, China
Keywords:
fluorescent
genome sequncing
Solanum lycopersicum.
Session: SAT06-5
Location: Hall of Ideas Room G/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 4:00 PM
Abstract ID:391 |
