| Abstract Detail
Conference Wide Imanishi, Shunsuke [1], Noguchi, Arisa [1], Nagata, Masayasu [1]. Analysis of tomato fruit mutants induced by gamma ray irradiation. FRUIT ripening is a complex, genetically programmed process. Identifying the components of this developmental switch is important for understanding the mechanism of fruit ripening. Mutational approaches have been highly instrumental for the study of the genetic and molecular bases of traits. In this study, we used a tomato cultivar Micro-Tom. It is well suited for large-scale mutagenesis and thus functional genomics owing to its small size and rapid life cycle. To obtain information on the genetic mechanism of fruit ripening, we screened for associated phenotypes and tried to identify loss-of-function mutations in some genes that involve in the process from mutant library induced by gamma ray irradiation. A population of 2,500 M2 families was visually phenotyped in the field and 266 mutations have exhibited variation from the wild type in one or more visually observable characteristics. Currently, 72 mutations have been checked the heritability, and 13 lines showed the same phenotype as described in the M2 generation. Now we start to monitor the differences of gene expression level by a cDNA macroarray technology.
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1 - National Institute of Vegetable and Tea Science (NIVTS), Department of physiology and quality science, 360 Kusawa, Ano, Tsu, Mie, 514-2392, JAPAN
Keywords:
tomato
Micro-Tom
mutant
gamma ray irradiation
color.
Session: Poster-155
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:387 |
