| Abstract Detail
Conference Wide Chen, Qin [1], Li, Haiyan [2], Beasley, D. [2], Bizimungu, B [2], Goettel, M. [2], Platt, H.W. (Bud) [3], DeKoeyer, D. [2], Murphy, A. [4]. Production of new somatic hybrids carrying both late blight and Colorado potato beetle resistance from Solanum polyadenium into cultivated potato by protoplast fusion. LATE blight and Colorado potato beetle (CPB) are the most disastrous disease and pest of potatoes. The development of resistant cultivars is a key component for the management of late bight and CPB. A Mexican wild species, S. polyadenium has been identified as a useful source of resistance to both pests. However, cross breeding through conventional methods is not possible due to sexual incompatibility barriers. Somatic hybridization via protoplast fusion provides an alternate approach to transfer the resistance from S. polyadenium. In this study, effective procedure was used to isolate large numbers of high quality of protoplasts from two accessions of the S. polyadenium (PI 230480 and PI 230463) and a 4x breeding clone (tbr 1) and a dihaploid clone (tbr 2) of S. tuberosum. Mesophyll protoplasts were isolated from in vitro plants and fusion was conducted using electrofusion method. Fused protoplasts were cultured in VKM medium with BSA or without BSA in dark at 20oC. The first cell division occurred on the fifth day. Regenerated plants were obtained from the calli cultured on both media after 4 months of culture. Cytology studies showed that the majority of regenerated plants had 48, a few has 72 chromosomes for combination of PI 230480 + tbr 2. The regenerated plants from combination of PI 230463 + tbr 1 have 48 chromosomes. Late blight and CPB resistance tests showed that all somatic hybrids derived from protoplasts of PI 230480 + tbr 2 were highly resistant to both late blight and CPB, indicating that late blight and CPB resistance in S. polyadenium has been successfully transferred into cultivated potato clones through somatic hybridization. However, most fused plants derived from PI 230463 + tbr 1 were susceptible to late blight and CPB. Molecular analysis using different DNA markers are in progress.
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1 - AAFC - Lethbridge Research Centre, P.O. Box 3000, Lethbridge, AB, T1J 4B1, CANADA
2 -
3 - Agriculture and Agri-Food Canada, 440 University Ave., Charlottetown, PE, C1A 4N6, Canada
4 - Agriculture and Agri-Food Canada, Potato Research Centre, P.O. Box 20280, 850 Lincoln Rd, Fredericton, NB, E3B 4Z7, Canada
Keywords:
Somatic hybridization
Protoplast fusion
S. polyadenium
S. tuberosum.
Session: Poster-152
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:376 |
