| Abstract Detail
Application of FISH in Support of Sequencing Plant Genomes - Afternoon Chang, Song-Bin [1], Stack, Steve [1]. Locating Centromeres on Tomato (Solanum lycopersicum) Linkage Maps Using FISH and a Centromere-Specific Probe. DETERMINING the location of centromeres on linkage maps of plants and animals is hampered by suppression of crossing over in centromeres and surrounding pericentric heterochromatin. Here we report a new and precise method for locating centromeres on the linkage map of tomato (Solanum lycopersicum) using TGR4, a 10 kb retrotransposon that localizes exclusively in the centromeres of all twelve tomato chromosomes. We hybridized a labeled TGR4 probe to filters carrying a tomato HindIII BAC library and identified 5542 BACs carrying the TGR4 sequence. Certain of these BACs carry mapped sequences that should reside at centromeres. This expectation is supported by two observations. First, markers in TGR4-containing BACs map near centromeres. Secondly, we demonstrate by FISH with CISS hybridization that a TGR4-positive BAC that also contains a marker from chromosome 1 localizes at the centromere of chromosome 1. Using this approach, we have identified a centromere-associated marker for eleven of the twelve tomato chromosomes. The centromere only for chromosome 9 could not be marked this way because a TGR4-containing BAC with a mapped marker on chromosome 9 was not identified. This novel technique should be applicable to other species where linkage maps and BAC libraries are available and a repeated sequence is known to reside exclusively at centromeres.
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1 - Colorado State University, Biology, Fort Collins, CO, 80523-1878, USA
Keywords:
tomato
linkage map
Solanum lycopersicum
centromere
FISH.
Session: SAT06-6
Location: Hall of Ideas Room G/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 4:30 PM
Abstract ID:36 |
