| Abstract Detail
Conference Wide Malyshev, Sergey V. [1], Grushetskaya, Zoya E. [2], Kartel, Nikolai A. [1], Kilchevsky, Alexander V. [3]. SSR-genotyping of tomato lines and varieties from Belarus collections. TO date, variety identification is usually carried out using morphological and physiological markers. A potential molecular marker system for DUS (distinctness, uniformity and stability) testing or for variety identification purposes needs to be highly reproducible and to possess a high discrimination power. Microsatellite markers for tomato have become available in increasing numbers and are a potentially useful system for the construction of large molecular-marker databases for tomato varieties. SSRs generally detect high levels of polymorphism, can detect heterogeneity and heterozygotes, and are amenable to automated analysis, detection and recording. The objective of this study was to assess genetic diversity within collection of tomato varieties that were commonly used in breeding programs in Belarus. A sample of 27 varieties and lines of tomato originating mainly from Belarus, Russia and Poland were analysed with a set of 15 microsatellite markers covering all chromosomes. Microsatellite polymorphism detected on the automated sequencer ALFexpress II was found to be relatively low. In total, 44 alleles were detected ranging from 1 (SSR48 and LELEUZIP) to 7 (TMS22), with an average of 3 alleles per marker and an average PIC of 0.377. Nevertheless, in the most cases the varieties had different microsatellite profiles. To reveal relationships between tomato genotypes, Nei’s genetic distances were calculated and the cluster analysis (NJ) was conducted. The results obtained provide new information about the relationships between the varieties analysed. They are useful for variety identification and for their utilization in further breeding programmes.
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Related Links:
Institute of Genetics and Cytology, NAS of Belarus
1 - Institute of Genetics and Cytology, NAS of Belarus, Molecular Genetics, Akademicheskaya st., 27, Minsk, 220072, Belarus
2 - Institute of Genetics and Cytology, NAS of Belarus, Functional Genetics of Plants, Akademicheskaya st., 27, Minsk, 220072, Belarus
3 - Institute of Genetics and Cytology, NAS of Belarus, Ecological Genetics and Biotechnology, Akademicheskaya st., 27, Minsk, 220072, Belarus
Keywords:
tomato
ssr
microsatellite
genetic diversity.
Session: Poster-150
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:356 |
