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Labate, J.A. [1], Robertson, L [1], Sheffer, S.M. [1], Wu, Feinan [2], Tanksley, Steve [2], Baldo, A.M. [1].

DNA Polymorphism Estimates Within Domesticated Tomato.

CULTIVATED tomato (Solanum lycopersicum L.) is known to have a narrow genetic base. This is in part due to population genetic processes such as founder events, genetic bottlenecks, and natural and artificial selection during domestication. We present empirical estimates of nucleotide polymorphism in 48 genes including COSII, EST-based, and several loci related to fruit quality traits. EST markers included five that were hypothesized to be cryptic wild species alleles (introgressions) within S. lycopersicum. All genes were resequenced in a diverse panel of 30 Plant Genetic Resources Unit (PGRU) tomato accessions, line TA496, and Solanum peruvianum accession G 32591. The majority of sampled tomato accessions represented the primary center of diversity (Peru, Chile, and Ecuador), and countries contiguous with the primary center. Original collections were made between 1932 and 1976. Within and between-species diversity estimates will be compared for the various marker types. These data will enhance our understanding of single nucleotide polymorphism (SNP) markers and the nature of genetic variation within cultivated tomato.


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Related Links:
PGRU seed crops genetic research


1 - US Dept. Agriculture, Agricultural Research Service, Plant Genetic Resources Unit, 630 W. North Street, Geneva, NY, 14456, USA
2 - Cornell University, Dept Plant Breeding and Genetics, Emerson Hall, Ithaca, NY, 14853, USA

Keywords:
SNP
Population Genetics
Solanum lycopersicum
Solanum peruvianum
COSII
EST.


Session: Poster-149
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:355


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