| Abstract Detail
Samokhvalova, Olga [1], Rakhimbaev, I.R. [2], Zhambakin, K.Zh. [2], Krasavin, V.F [3]. Breeding of new potato varieties by using dihaploid selection technology. CROSSING S. Tuberosum that has tetraploid set of chromosomes (2n=48) with dihaploid wild varieties (2n=24) and development of new varieties is an important approach in plant selection science and agricultural industry. This is because other existing techniques, i.e. crossing with wild varieties and direct crossing between varieties with different chromosome sets are problematic: the former suffers from experimental difficulties, while the latter usually produces sterile hybrids. The goal of the presented research is to obtain dihaploids of S. Tuberosum by androgenesis technique and develop new potato varieties with valuable traits. As a result of the research, the laboratory protocol of volume production of potato dihaploids was prepared. In addition, we have reached new conclusions regarding morphogenesis and regeneration of dihaploid plants in anther culture of potato. Potato clone multiplication technique and microtube development stimulation technique both in vitro and in vivo were developed. Those techniques allow to optimize the technology of volume production of dihaploid plants. The new findings in studies of physiological processes of flowering and tube-forming of potato allow to establish practical guidelines for improvement of elite seed production. The new collection of dihaploid potato varieties obtained within our research is kept at in the Laboratory of Potato Selection of the Research Institute of Agriculture of Potato and Vegetables (Kainar City, Kazakhstan). At this Institute, experimental work on crossing dihaploid with wild varieties is being performed, and new seed lines with valuable traits are being obtained.
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1 - Allan Hancock College, Biology, 800 South College Drive, Santa Maria, CA, 93454, USA
2 - Institute of Physiology, Genetics and Bioengineering, 45 Timiryazev Str., Almaty, 050060, Kazakhstan
3 - Research Institute of Agriculture of Potato and Vegetables, Potato Selection, Institute str, Kainar, 060040, Kazakhstan
Keywords:
Potato
S. tuberosum
anther culture
dihaploid.
Session: Poster-147
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:352 |
