| Abstract Detail
Conference Wide Xiao, Han [1], Meulia, Tea [2], Welty, Nicholas, D [3], van der Knaap, Esther [1]. Role of SUN in controlling tomato fruit shape and expression of candidate genes. THE tomato fruit shape locus sun was identified as a major QTL on chromosome 7 in a cross between the Solanum lycopersicum Sun1642 and a wild relative, S. pimpinellifolium accession LA1589. The Sun1642 allele of sun produces elongated fruit, while the LA1589 allele of sun produces round fruit. Near Isogenic Lines (NILs) in the LA1589 background are used to study the effects of sun on plant and fruit phenotype. The maximal difference in fruit shape occurs 10 days after anthesis, coinciding with the heart stage of embryo development. This indicates that sun acts mainly after anthesis, during cell division and initial cell enlargement stage of tomato fruit development. In addition, sun has little or no effect on other fruit traits such as mass and seed number per fruit. Gene action at sun is additive and suggests that sun is the result of a gain-of-function mutation acting in a dosage-dependent manner. Thus, we expect that the SUN candidate gene is differentially expressed. Positional cloning of SUN revealed five likely candidate genes: CDS1, CDS2, CDS3, CDS4, and CDS5. CDS1 and CDS5 are differentially expressed in flowers and fruits harboring different sun alleles. Transcript levels for the three remaining candidate genes are either undetectable or inconsistent upon replication. The transcript levels of CDS1 and CDS5 in developing fruits from homozygous and heterozygous plants also support the hypothesis that these genes may underlie sun acting in a dosage-dependent manner. Transcript levels of CDS1 and CDS5 during fruit development indicate changes in expression pattern after pollination and mirror the observed changes in fruit shape. This work is supported by a grant from NSF DBI 0227541
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1 - The Ohio State University, Horticulture and Crop Science, 1680 Madison Ave., Wooster, OH, 44691
2 - The Ohio State University, Molecular and Cellular Imaging Center, 1680 Madison Ave., Wooster, OH, 44691, USA
3 - The Ohio State University, Horticulture and Crop Science, 11444 Eby Rd., Sterling, OH, 44276, USA
Keywords:
tomato
QTL
Fruit shape
SUN.
Session: Poster-146
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:349 |
