| Abstract Detail
Physiology Giorno, Filomena [1], Massarelli, Immacolata [1], Nurcato, Roberta [1], Barone, Amalia [2], Grandillo, Silvana [1], Alba, R.M. [3], Giovannoni, Jim [3], Monti, Luigi [1], Leone, Antonella [4], Grillo, M.Stefania [1]. Tomato fruit set at high temperature: linking physio-biochemical adjustments to changes in gene expression. HIGH temperature affects dramatically all stages of tomato flower development, especially anthesis, causing poor fruit set and, ultimately, yield loss. By comparing two tomato genotypes with a diverse ability to set fruit under heat stress (HS) (cv. SALADETTE, heat-tolerant and cv. PULLREX, heat-sensitive), a repertoire of differentially expressed genes was created by microarray analysis and Suppression Subtractive Hybridization (SSH) (Massarelli et al., 2005). A more accurate comparison of the changes in gene expression occurring at different stages of flower development under control (28°C) or heat shocked (36°C) plants of the two contrasting tomato genotypes was conducted by qRT-PCR. The subset of genes analyzed included genes known to encode proteins involved in several aspects of tomato flower development (Lat59, Qrt3, LTP protein, 108 Protein, Male-Sterility Protein), HS-proteins (Hsp17.4 CII, ERD15, Cytocrome P450, Wound-induced Proteinase inhibitor, Hsp101, Lea5), transcription factors (WRKY, HsfA2) or encoding enzymes for the synthesis of soluble sugars (AGPase, beta-fructofuranosidase, DAHP synthetase 2, UDP-glucose pyrophosphorylase). Moreover, genotype- and stage-specific differences were found for some physiological and biochemical aspects (pollen viability and germination, content in soluble sugars and polyamines) monitored at different stages of flower developmental of the two tomato genotypes under HS or control temperature. These results will be discussed altogether in an attempt to find a causal relationship between changes in gene expression and physio-biochemical adjustments occurring at high temperature in developing tomato flowers and, ultimately, affecting the ability to set fruit. This work was supported by MIUR Project FIRB PlantSTRESS Reference: Massarelli I.,Giorno F., Barone A., Grandillo S., Giovannoni JJ., Monti L., Leone A., Grillo S. 2005 in "2nd Solanaceae Genome Workshop" September 25-29, Ischia-Italy L12, p. 44.
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1 - Cnr-Igv, Institute of plant genetics, Research Division of Portici, Via Università, 133, Portici, Italy, 80055, Naples
2 - University of Naples Federico II, Department of Soil, Plant and environmental Sciences, Via Università 100, Portici (NA), Italy, 80055
3 - The Boyce Thompson Institute for Plant Research and U.S. Department of, Cornell University, Tower Rd., Ithaca, NY, 14853, USA
4 - University of Salerno, Department of Pharmacological Sciences, Via Ponte don Melillo, Fisciano, Italy, 84084, Salerno
Keywords:
cDNA-microarrays
Quantitative Real Time PCR (QRT-PCR)
heat stress
tomato flower development
fruit set.
Session: Poster-19
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:340 |
