| Abstract Detail
Conference Wide Gorguet, Benoit [1], van Heusden, Adriaan W. [1], Lindhout, Pim [1]. Towards cloning of a positional sterility gene in tomato: Fine mapping of ps-2 on the short arm of Chromosome 4. AUTO-STERILITY in tomato (S. lycopersicum) can be divided in two groups: male sterility and functional sterility. The use of male sterility genes in tomato breeding programs is of little value due to the difficulties of maintaining the male sterile lines. Functional sterility is characterized by a strong restriction of natural pollination due to abnormal morphology and function of the flower, while the pollen present in the anthers is viable. Positional sterility 2 (ps-2) is the result of a spontaneous mutation in the Czech cultivar “Vrbicanske nizke” and is characterized by non-dehiscent anther bags. In order to map the ps-2 gene we developed an F2 segregating population from the cross between a ps-2 line (S. lycopersicum) and S. pimpinellifolium accession GI 1554. The latter was used as wild type donor because the level of DNA polymorphism is substantially higher in an inter-specific segregating population which is essential for the development of molecular markers. AFLP markers closely linked to the ps-2 locus were identified by BSA and a local genetic linkage map was constructed with SSR markers, AFLP markers and RFLP derived CAPS markers. The ps-2 gene mapped between the CAPS markers CT192 and CT175 on the short arm of Chromosome 4. In addition, a recombinant population was developed by screening 1880 F2 plants for recombination between the two flanking CAPS markers CT175 and CT192. About 145 recombinant plants were identified. Additional COS derived CAPS markers were developed and mapped on the local genetic linkage map. Eventually we mapped the ps-2 locus in a window of 1.5 cM delimited by two CAPS markers. These two closest CAPS markers flanking the ps-2 locus are currently being used as a starting point for the map based cloning of the ps-2 gene.
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1 - Wageningen University, Laboratory of Plant Breeding, P.O Box 386, Wageningen, 6700AJ, The Netherlands
Keywords:
tomato
functional sterility
mapping.
Session: Poster-142
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:339 |
