| Abstract Detail
Conference Wide Gorovots, Rena [1], Akad, Fouad [1], Eybishitz, Assaf [1], Bar-Or, Carmiya [2], Koltai, Hinanit [2], Vidavski, Favi [1], Czosnek, Henryk [1]. Characterization of resistance of tomato to Tomato yellow leaf curl virus (TYLCV): expression of stress response genes upon virus infection and abiotic stresses. THE whitefly-transmitted TYLCV is a major constraint to tomato production. Two nearly isogenic tomato lines, susceptible (S) and resistant (R) to TYLCV (with resistance introgressed from S. habrochaites) have been developed. Based on the assumption that resistance to virus and to various abiotic have common determinants, we have compared the expression of two key gene families involved in early response to virus infection and various abiotic stresses: mitogen activated protein kinases (MAPKs) and heat shock proteins (HSPs). Microarray profiling of gene expression in S vs. R lines (before and after whitefly-mediated virus inoculation) using the TIGR potato microarray indicated that a significant decrease in expression level of HSP and MAPK genes was detected 7 days post TYLCV inoculation, more pronounced in S than in R. Hence the early response to virus inoculation is accompanied by a decline in the abundance of HSP and MAPK transcripts. To validate these results cDNA libraries of S and R before and after inoculations have been constructed. Transcripts of differentially expressed genes are under analysis. Antibodies against MAPKs and HSPs have been used to recognize changes in protein patterns related to viral infection and to several abiotic stresses. All detected MAPKs and HSPs reflected a similar response upon inoculation by whiteflies with/without TYLCV, visualized as a decrease in the abundance of the reactive protein during the first 5 to 7 days post inoculation. This decrease is less dramatic in R than in S tomatoes. Similar changes in patterns are valid for several kinds of abiotic stresses: H2O2, NaCl, ethanol, heat, cold, and salicylic acid. From these studies we conclude that R plants possess a higher buffering capacity than S plants to biotic and abiotic changes and that the plant response to these two kinds of stress may involve overlapping pathways.
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1 - The Hebrew University of Jerusalem, The Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food and Environmental Qua, Rehovot, 76100, Israel
2 - Volcani Center ARO, Ornamental Horticulture, Bet Dagan, 50250, Israel
Keywords:
Begomovirus
Resistance
abiotic stress
biotic stress
MAPK
HSP.
Session: Poster-139
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:336 |
