| Abstract Detail
Application of FISH in Support of Sequencing Plant Genomes - Afternoon Jiang, Jiming [1]. The FISHing tools in a genome sequencing project. FLUORESCENCE in situ hybridization (FISH) is a technique to map DNA sequences on cytological specimens. FISH has become a valuable tool in sequencing projects of species with large complex genomes. FISH is the most efficient way to anchor a bacterial artificial chromosome (BAC) clone to a specific chromosome and to associate a DNA fragment with a specific chromatin feature. All of the currently available BAC fingerprinting and DNA sequencing technologies will inevitably result in physical gaps. The size information of the gaps is critical for designing appropriate strategies to eventually close the gaps. FISH-based approach has been proved to be the most effective methodology to estimate the sizes of such gaps. Other applications of FISH in genome sequencing will be discussed.
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1 - University of Wisconsin - Madison, Horticulture, 1575 Linden Drive, Madison, WI, 54501, United States
Keywords:
FISH
Cytogenetic mapping
genome sequencing.
Session: SAT06-2
Location: Hall of Ideas Room G/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 2:15 PM
Abstract ID:326 |
