| Abstract Detail
Breeding and Genetics Marfil, Carlos F. [1], Camadro, Elsa L. [2], Masuelli, Ricardo [1]. Phenotypic instability and epigenetic variability in a population of the wild potato Solanum ruiz-lealii. THE wild potato species S. ruiz-lealii (2n=2x=24), a putative hybrid between S. kurtzianum and S. chacoense, is endemic to Mendoza province, in Argentina. Recurrent flower malformations, that varied among inflorescences of the same plant, were observed in plants of a natural population. These abnormalities could be result of: genomic instabilities, nucleus-cytoplasmic incompatibility or epigenetic changes. The nuclear, mitochondrial and methylation status of plants with normal and abnormal flower phenotypes were analyzed by AFLPs, restriction analysis and MSAP, respectively. The AFLPs and the restriction patterns of the mitochondrial DNA did not differentiate the stable from the unstable phenotypes. However, the phenogram obtained with the DNA methylation data discriminated the normal and aberrant phenotypes into two different groups, indicating that unstable phenotypes have a similar global methylation status that was different to the status of normal plants. In addition, the intra-population variability regarding DNA methylation was greater that the genomic sequence variability. The importance of considering this source of variability in natural populations and investigating how modifications in DNA methylation could induce flower abnormalities is established.
Log in to add this item to your schedule
1 - Laboratorio de Biología Molecular, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, CONICET, INTA EEA La Consulta, Alte. Brown 500, Chacras de Coria, Mendoza, M5528AHB, Argentina
2 - Unidad integrada EEA Balcarce, INTA - FCA - UNMdP, CONICET, agronomía, c. c. 276, Balcarce, Buenos Aires, 7620, Argetnina
Keywords:
Solanum ruiz-lealii
flower malformation
DNA methylation.
Session: Poster-28
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:325 |
