| Abstract Detail
Translational Genomics - Morning Brewer, Marin [1], Moyseenko, Jenny [1], Monforte, Antonio [2], van der Knaap, Esther [1]. Phenotypic variation in three tomato (Solanum lycopersicum) populations: identification and comparison of principal components and quantitative trait loci controlling fruit morphology. THE fruit of tomato display a variety of shapes and sizes, from small and round like the wild relative Solanum pimpinellifolium to large and elongated as seen in two domesticated varieties, S. lycopersicum Howard German (HG) and Banana Legs (BL). In addition, fruit from HG are tapered and pointed at the distal end, whereas fruit from BL are rounded and often indented with or without a protrusion. To understand the molecular mechanisms responsible for this variation, a software application Tomato Analyzer was used to measure several fruit morphology attributes in two F2 populations and a BC1 population derived from HG or BL crossed with S. pimpinellifolium. Principal components analysis was conducted across all traits within and between populations. Molecular linkage maps were developed and subsequent QTL analysis of the first three principal components (PC) and individual attributes demonstrated that multiple QTL were contributing to this variation in fruit morphology. Furthermore, the first three principal components were dissected into individual attributes to demonstrate which traits were contributing to the greatest amount of variation. The sun locus on chromosome 7 contributed most to one of the PC and several shape attributes in all populations. Also, one PC, which was largely affected by heart shape and distal end indentation area, clearly distinguished the BL and HG F2 populations. Interestingly, QTL of this PC mapped to different locations in the HG and BL populations, which could explain differences in shape observed in the parental fruit. Furthermore, QTL for the distal end shape attributes blockiness and angle mapped to different locations in the two populations. QTL on chromosome 7 and 11 were detected only in HG, whereas QTL on chromosomes 2, 6, and 12 were detected only in BL. In addition, many small effect loci were consistently detected across the populations. Funded by NSF-DBI 0227541
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Related Links:
Lab webpage
1 - Ohio State University (OARDC), Horticulture and Crop Science, 1680 Madison Avenue, Wooster, Ohio, 44691-4096, USA
2 - Laboratori CSIC-IRTA de Genética Molecular Vegetal, Barcelona, , Spain
Keywords:
tomato
shape
QTL.
Session: SAT05-6
Location: Hall of Ideas Room I/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 10:15 AM
Abstract ID:321 |
