| Abstract Detail
Conference Wide Wu, Feinan [1], Tanksley, Steve [2]. Comparative analyses between tomato single copy region and Arabidopsis duplicated counterparts. TOMATO (Euasterid) and Arabidopsis (Eurosid) diverged more than 94 million years ago. Studies have indicated multiple genome duplication events in Arabidopsis and one of them occurred after their divergence. Understanding of this complex duplication history in Arabidopsis and comparative studies between the two genomes will facilitate the use of Arabidopsis as a genetic and genomic model for tomato and other Euasterid species. We have computationally screened Arabidopsis CDS database and Euasterid unigene sets, and developed a set of 2869 single copy, conserved orthologs (COSII genes) common to most, if not all, euasterid species as well as Arabidopsis (Wu et al submitted). Up to date, more than 400 COSII markers have been mapped on the high-density tomato map. At the macrosynteny level, only small syntenic regions (<10cM) could be observed, which is consistent with previous study (Fulton et al 2002). Interestingly, the single copy Arabidopsis orthologs for the two adjacent tomato markers (0.6cM apart), are located in the duplicated regions at chromosome 2 and 3, respectively. The result suggested that tomato genome, but not Arabidopsis genome, may resemble the ancestral genome in terms of gene order; The lack of synteny between the two genomes could result from the gene loss in one of the duplicated regions in Arabidopsis followed by rearrangements within and between chromosomes. We expect to observe an increased level of synteny between the ancestral Arabidopsis genome (before the duplication event) and the current tomato genome. By continuing examination of the entire tomato map using COSII markers and by connecting to their Arabidopsis counterparts, we may be able to depict more clearly the evolution of the two model plants.
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Related Links:
single copy, orthologous gene markers - COSII marker
1 - Cornell University, Dept Plant Breeding and Genetics, Emerson Hall, Ithaca, NY, 14853, USA
2 - Cornell University, Plant Breeding and Genetics, 248 Emerson Hall - Cornell University, Ithaca, NY, 14853, USA
Keywords:
COSII markers
tomato-Arabidopsis
comparative genomics
Genome evolution.
Session: Poster-134
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:314 |
