| Abstract Detail
Conference Wide Tam, Sheh May [1], Samipak, Sompid [1], Chetelat, Roger [2]. The roles of the mismatch repair genes MSH2 and MSH7 in homeologous recombination in tomato. MISMATCH repair genes play important roles in ensuring genetic stability and inhibiting genetic recombination between divergent DNA sequences. Genetic stocks developed by the TGRC, namely monosomic addition, substitution and introgression lines (MAs, SLs and ILs), in which observed rates of homeologous recombination are strongly suppressed, are being used as a model system to study the roles of MSH2 and MSH7 in meiotic recombination and hybrid infertility. Lines with reduced expression of MSH2, produced by co-suppression or RNAi, were crossed with tomato substitution and introgression lines containing chromosome 10 (SL-10) or a recombinant chromosome 7 (SL-7F) from the wild nightshade (Solanum lycopersicoides). We also isolated MSH7 from tomato, which will be used for further plant transformations and gene silencing by similar methods. We present on-going experiments and preliminary results regarding the effects of MSH2 expression on homeologous recombination rates in the tomato x nightshade system.
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1 - University of California Davis, Plant Sciences, 151, Asmundson Hall, One Shields Avenue, Davis, CA, 95616, USA
2 - Univ. of California, Dept. of Plant Sciences, C.M. Rick Tomato Genetics Resource Center, Davis, CA, 95616, USA
Keywords:
mismatch repair genes tomato nightshade.
Session: Poster-133
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:306 |
