| Abstract Detail
Application of FISH in Support of Sequencing Plant Genomes - Afternoon Chang, Song-Bin [1], Royer, Suzanne [1], Anderson, Lorinda [1], Stack, Steve [1]. Evaluation of Fluorescence In-Situ Hybridization (FISH) technique in supporting tomato (Solanum lycopersicum) genome sequencing project. WE are doing FISH to support the tomato sequencing project. For this, we hybridize DNA from the HindIII BAC library to demonstrate its location on pachytene chromosomes. This is done to help confine the sequencing effort to euchromatin where most genes are located. So far, many of the BACs used are seed BACs with mapped markers that were thought likely to reside in or near telomeres or pericentric heterochromatin. The success rate for locating BACs in gene-rich euchromatin is higher than the success rate for locating BACs in gene-poor heterochromatin. The reason is that BACs in heterochromatin and BACs near euchromatin-heterochromatin borders are often rich in repetitive sequences that hybridize to multiple sites in heterochromatin and/or telomeres. Even when BACs hybridize to single sites, the results may be unexpected. For example, 1) BACs may localize to chromosomes or chromosome arms that are different from their mapped locations, 2) BACs may be localized in different orders than their mapped arrangements, and 3) BACs may be separated by physical distances that are out of proportion to their map separations. Such results emphasize the importance of FISH to the sequencing effort in tomato.
Log in to add this item to your schedule
1 - Colorado State University, Biology, Fort Collins, CO, 80523-1878, USA
Keywords:
FISH
tomato
BACs
euchromatin
heterochromatin.
Session: SAT06-4
Location: Hall of Ideas Room G/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 3:30 PM
Abstract ID:300 |
