| Abstract Detail
Physiology McCue, K.F. [1], Allen, P.A. [1], Shepherd, L.V.T. [2], Blake, A. [2], Novy, Richard [3], Stewart, D. [2], Davies, H.V. [2], Belknap, W.R. [1]. Manipulation and compensation of steroidal glycoalkaloid biosynthesis in potatoes. STEROIDAL glycoalkaloids (SGAs) are undesirable secondary metabolites produced in Solanaceous plants including, potato, tomato and eggplant. Two tri-glycosylated alkaloids, α-chaconine and α-solanine occur naturally in potato tubers and can accumulate to excessive levels due to mechanical, environmental and genetic perturbations. We have identified members ot the SGA glycosyl transferasese gene family coding the UDP-galactose:solanidine galactosyltransferase (Sgt1), the UDP-glucose:solanidine glucosyltransferase (Sgt2), and the UDP-rhamnose:β-solanine/β-chaconine rhamnosyltransferase (Sgt3). Reverse genetic manipulation of the Sgt gene family members using transgenic lines expressing acitve antisense gene constructs results in a shift of SGA pathway flux resulting in compensation by other pathway intermediates and products. New Sgt gene family members are being studied to verify the substrate specificity and biosynthetic sequence. Additional studies are underway to use the members of this gene family to completely block accumulation of SGAs in tubers.
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1 - United States Department of Agriculture, Agricultural Research Service, Crop Improvement and Utilization Research Unit, 800 Buchanan Street, Albany, CA, 94710, USA
2 - Scottish Crop Research Institute, Quality, Health & Nutrition Programme, Invergowrie,, Dundee, DD2 5DA, UK
3 - United States Department of Agriculture, Agricultural Research Service, 1693 South 2700 West, Aberdeen, Idaho, 83210, United States
Keywords:
Solanum tuberosum
Potato
glycoalkaolids
chaconine
solanine
Secondary metabolism.
Session: PAA05-1
Location: Hall of Ideas Room F/Monona Terrace
Date: Thursday, July 27th, 2006
Time: 8:30 AM
Abstract ID:298 |
