| Abstract Detail
Breeding and Genetics McCord, P.H. [1], Yencho, G.C. [2], Clough, M. [3], Sosinski, B.R. [2], Haynes, Kathleen G. [4]. Development of molecular markers for resistance to internal heat necrosis (IHN). INTERNAL heat necrosis (IHN) is a physiological disorder of potato tubers resulting in the death and discoloration of parenchymal tissue. Environmental conditions and the large acreage of the IHN-susceptible cultivar ‘Atlantic’ result in significant losses due to the disorder in the mid-Atlantic states and Florida. As previously reported, a combination of bulk segregant analysis and AFLP marker technology was used to search for molecular markers for IHN in a small population of tetraploid 4x-2x Solanum tuberosum x S. phureja-S. stenotomum (tub x phu-stn) hybrids. These clones are being used to breed an IHN-resistant, high specific-gravity replacement for ‘Atlantic’. Five putative markers from this initial study were then tested on a much larger population of 320 clones from a combining ability study for IHN resistance/susceptibility. Two of these markers were highly significantly associated with resistance/susceptibility to IHN, but together explained only 8.6% of the variation for IHN incidence. Clearly, better markers are needed if they are to prove useful in marker-assisted selection and/or identification of candidate genes. We are currently undertaking a multifaceted approach to understanding the genetics of IHN. We will report the progress of our work in the following two areas: a repeated search for AFLP markers via bulk segregant analysis with more individuals per bulk; and QTL mapping in two distinct populations using SSR and AFLP markers.
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1 - North Carolina State University, Horticultural Science, Campus Box 7609, Raleigh, NC, 27695, U.S.A.
2 - North Carolina State University, Horticultural Science, Campus Box 8619, Raleigh, NC, 27695, U.S.A.
3 - North Carolina State University, Horticultural Science, Vernon G. James Research and Extension Center, Plymouth, NC, 27962, U.S.A.
4 - United States Department of Agriculture/Agricultural Research Service, Vegetable Laboratory, 10300 Baltimore Ave., Beltsville, MD, 20705
Keywords:
QTL mapping
internal heat necrosis.
Session: PAA09-3
Location: Hall of Ideas Room G/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 2:00 PM
Abstract ID:296 |
