| Abstract Detail
Pepper - Afternoon Jung, Min [1], Lee, Yun H [1], Shin, Sun H [1], Yoon, Jin S [1], Choi, Soon H [1], Shim, Dong Bo [1], Harn, Chee H [1]. A breakthrough for Pepper Transformation. PEPPER is known as a difficult plant to transform and has resisted the efforts of many laboratories for many years. We have developed a transformation system by inducing callus and shoot before and after co-culturing with Agrobacterium, respectively. It is called as callus induced transformation (CIT) and the transformation rate was ranged from 0.5-1.0% depending on the genotype. This method has been worked for many different inbred lines including chilli and paprika, and based on data the CIT turns out a stable and reliable method. On top of this method, we have also developed a multi-transformation technique to enhance the number of transformed pepper in the same experiment period. Pepper explant was transformed by the same Agrobacterium strain (EHA105) independently containing different vectors in which different genes were inserted. By doing this way, in a given period, more chance of obtaining differently transformed pepper was available comparing to the co-culturing with one kind Agrobacterium with one vector.
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Keywords:
transformation
Pepper
callus.
Session: SAT08-8
Location: Hall of Ideas Room H/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 4:30 PM
Abstract ID:269 |
