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Abstract Detail


Coffee Genomics - Morning

Moncada, Pilar [1], Montoya, Juan C. [1], Lopez, german [1], Gonzalez, Alexandra [1], Iriarte, Gloria [1], Zarate, Luz A. [1], Cristancho, Marco [1].

Advances in construction of tetraploid and diploid maps and in populations for qtl analysis in coffee?

FOR map construction, a segregating population with its parents, and polymorphic markers are required. In perennial crops as coffee the development of a population is a long process. So, in order to construct a coffee map, two populations were developed in parallel. The first is an F2 tetraploid population with 300 individuals developed from the cross between Caturra variety and Etiopia 42. This population has shown very low polymorphism (65%) when evaluated with SSRs. In order to construct the maps different markers have been used. Among them, microsatellites are the most successful ones. COS markers obtained from Hedyotis and from Coffea have shown low polymorphism in both populations. Different strategies have been used in order to map EST sequences. Among them, CAPs and SNPs markers are being developed. These maps will be very useful tools to speed up the selection in a perennial crop such as coffee (Coffea spp.), allowing the use of marker assisted selection after mapping the QTL of traits of interest. In the Colombian coffee breeding program those traits are: resistance to coffee leaf rust, resistance to coffee berry borer, beans characteristics, yield etc. At this time, we are developing different populations for QTL analysis using genotypes contrasting for those traits as parents.  Financial support from FEDERACAFE and the Ministry of Agriculture of Colombia.


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1 - Cenicafe, Plant breeding, Apartado aereo # 2427, Manizales, Caldas, , Colombia

Keywords:
mapping
Molecular markers
COSII markers
coffee.


Session: SAT01-6
Location: Hall of Ideas Room G/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 8:40 AM
Abstract ID:260


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