| Abstract Detail
Translational Genomics - Morning Rehfeld, K [1], Rensink, WA [1], Hart, A [1], Liu, J [1], Ly, E [1], Buell, CR [1]. Gene expression profiling of the potato life cycle. COMPARED to other plants, potato contains a number of unique developmental features, most importantly tuber development. To provide insight in the complete life cycle of potato in all its developmental stages, we have generated expression profiles of 16 distinct tissues of potato at different developmental stages. As most potato plants are grown from seed tubers we have used the true seed potato variety Gilroy to study early seedling stages and development. RNA samples were isolated from a wide range of developmental stages including leaf (young and old), shoot, etiolated seedling, petiole, flower, bud, corolla, stamen, pistil, stolon, tuber, microtuber, root, berry, and tuber epidermis. After in vitro amplification the RNA was hybridized to a potato cDNA microarray containing ~12,000 unique clones representing ~10,000 transcripts. A total of four biological replicates were collected and a loop design was used for the hybridizations which will enable us to detect differential expression among all stages of development. We are currently in the process of completing the hybridizations. We will report on the identification of the differential expression during the life cycle of potato and the unique features of each tissue compared to other tissues. All expression data will be made available through the Solanaceae Gene Expression Database (http://www.tigr.org/tdb/potato/). This collection of expression profiles will increase our understanding of potato development and could facilitate research on improving potato production.
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1 - The Institute for Genomic Research, 9712 Medical Center Dr, Rockville, MD, 20850, US
Keywords:
expression profiling
atlas
encylopedia.
Session: SAT05-9
Location: Hall of Ideas Room I/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 11:15 AM
Abstract ID:26 |
