| Abstract Detail
Tobacco - Morning Opperman, Charles [1], Lommel, Steve [1], Burke, Mark [1], Bedell, Joey [2], Budiman, Arief [2]. The tobacco genome initiative: gene discovery and data mining in Nicotiana tabacum. ALTHOUGH cultivated tobacco, Nicotiana tabacum, is of great economic significance, relatively little information was previously available about its genome structure and organization. N. tabacum has a very large genome size compared with other cultivated solanaceous plants. At approximately 4.5 billion base pairs, it is 1.5 times the size of the human genome. As with the human genome, the vast majority of these base pairs occur as highly repetitive non-coding sequence. We are have utilized a combination of strategies to identify a large percentage of genes in N. tabacum. We employed a methyl filtration approach to identify gene-rich regions in N. tabacum in order to expedite the gene discovery. To date, we have sequenced 1,700,000 lanes of methyl filtered clones and observe a dramatic increase in gene discovery in filtered vs. non-filtered libraries. We have also performed EST sequencing from various Nicotiana libraries and, to date, have sequenced over 80,000 ESTs. Genes tagged using these two strategies have been used as probes to identify BAC clones for more targeted sequencing. Numerous BACs have been sequenced, revealing information about both gene structure and genome organization in Nicotiana spp.
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1 - NC State University, College of Agriculture and Life Sciences, Raleigh, NC, 27695, USA
2 - Orion Genomics, LLC, St. Louis, MO, 63108, USA
Keywords:
gene discovery
data mining.
Session: SAT03-1
Location: Hall of Ideas Room H/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 8:00 AM
Abstract ID:256 |
