| Abstract Detail
Tuesday Morning Contributed Talks Litt, Amy [1], Pan, Irvin [2], McElhinny, Elaine [1], McQuinn, Ryan [3], Giovannoni, Jim [4], Irish, Vivian [5]. Comparative morphological and transcriptome analysis of fruit development in tomato and flowering tobacco (N. sylvestris). WE have initiated a combined morphological and transcriptomic analysis of fruit development in Solanaceae aimed at identifying genes common to fruit development across Solanaceae as well as genes that play divergent roles in the development of dry and fleshy fruits. We have begun with a description of the morphological development of the Nicotiana sylvestris capsule, from anthesis through to dehiscence, and have identified benchmark stages comparable to those identified in post-fertilization tomato development for molecular analyses. We are also analyzing morphological development in Petunia, which, along with Nicotiana, has an ancestral capsule, as well as two species with independent origins of capsules (Datura) and berries (Cestrum). At the same time we are performing comparative transcriptome analyses of fruit development in these species, using the 12,000 unigene TOM2 microarray. Our initial experiments with 2 dpa fruits of tomato and N. sylvestris demonstrate that this technique is effective in detecting a large number of genes that are expressed at similar levels in both species, as well as significant numbers of genes expressed differentially in tomato or flowering tobacco. Preliminary analyses of transcripts whose expression is correlated with particular fruit types or are species specific will be presented.
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1 - New York Botanical Garden, 200th St. and Southern Blvd., Bronx, NY, 10458, USA
2 - Yale University, Department of Molecular, Cellular, and Developmental Biology, New Haven, CT, 06520, USA
3 - USDA-ARS and Boyce Thompson Institute for Plant Research, Tower Rd., Cornell University campus, Ithaca, New York, 14853, United States of America
4 - The Boyce Thompson Institute for Plant Research and U.S. Department of, Cornell University, Tower Rd., Ithaca, NY, 14853, USA
5 - Yale University, Molecular, Cellular and Developmental Biology, 266 Whitney Ave, PO Box 208104, New Haven, CT, 06520, USA
Keywords:
comparative fruit development
morphology
transcriptome
tomato
Nicotiana.
Session: SOL04-8
Location: Ballroom AB/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 10:50 AM
Abstract ID:255 |
