| Abstract Detail
Breeding and Genetics Brown, C.R. [1], Crosslin, James [2], Mojtahedi, Hassan [3], James, Steven [4], Charlton, Brian [5]. Stability and Nature of Resistance to Corky Ringspot Disease in an Advanced Tetraploid Breeding Population of Potato. CORKY ringspot (CRS) disease of potato in the Pacific Northwest of the United States is a viral disorder showing symptoms on the surface, and internally. It is caused by the tobacco rattle virus (TRV) which is transmitted by the stubby root nematode. The vector is quite common in the Columbia Basin of Washington and Oregon. However, viruliferous nematodes are rarer, infesting 5-10% of fields. Eradication of the vector appears to be impossible, and sequence of commonly adopted crop rotation may prepotentiate it. Wheat and corn preceding potato has been found to increase the incidence of CRS disease in the potato. Controlled by soil fumigation at present, the development of new cultivars with resistance is an attractive solution, if resistance is durable and does not provide viral inoculum from symptomless apparently resistant latent carriers. We present data which indicates resistance in a group of clones is stable at two distinct locations over several years of testing. Detection of TRV was attempted in symptomatic susceptible and asymptomatic “resistant” clones in tuber tissue using reverse transcriptase polymerase chain reaction (RT-PCR). TRV was rarely detected in symptomless resistant cultivars. A strong though not perfect correspondence of RT-PCR detection with occurrence of symptoms was found in susceptible materials. Therefore, resistance appears to be resistance to viral infection. These results indicate that the breeding program has successfully identified clones which are not damaged by TRV infection and which are free of virus infection, meaning they will not serve as a source of inoculum.
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Related Links:
USDA/ARS, Prosser, WA website
1 - US Dept. of Agriculture/ARS, Prosser, WA, 99350, USA
2 - United States Department of Agriculture, Agricultural Research Service, Forage and Vegetable Crop Research Unit, 24106 N. Bunn Road, Prosser, WA, 99350, USA
3 - Usda/Ars, 24106 N. Bunn Rd., Prosser, WA, 99350, USA
4 - Oregon State University, Central Oregon Ag Research Center, 850 NW Dogwood Lane, Madras, OR, 97741, USA
5 - Oregon State University, Klamath Experiment Station, 6941 Washburn Way, Klamath Falls, OR, 97603, USA
Keywords:
Tobacco rattle virus
Solanum
Paratrichodorus allius
polymerase chain reaction
disease resistance screening
nematode vector.
Session: PAA09-9
Location: Hall of Ideas Room G/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 4:00 PM
Abstract ID:253 |
