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Abstract Detail


Physiology

Rensink, WA [1], Rehfeld, K [1], Hart, A [1], Liu, J [1], Ly, E [1], Buell, CR [1].

Analysis of abiotic stress responses in potato, Solanum tuberosum, by comparative and functional genomics approaches.

TEMPERATURE, both high and low, and salinity are common stress conditions in plants and affect both plant growth and crop production. To identify abiotic stress response genes as well as to gain insight in the overall transcriptional responses, we have performed a series of experiments using potato as a representative for the Solanaceae. Using a cDNA spotted microarray containing ~12,000 potato clones we have collected a large number of expression profiles for potato under abiotic stress conditions. Potato plants were grown under controlled conditions and heat, cold or salt stress was applied. Samples were collected at three time-points (3, 9 and 27 hours) both from leaves and roots. A total of 3,314 clones could be identified as significantly up- or down-regulated in response to at least one stress condition. The genes represented by these clones encode transcription factors, signal transduction factors, and heat-shock proteins. Natural variation in heat stress tolerance was exploited using alternative potato germplasm. A broad range of wild species of potato is available from the USDA germplasm collection that has been characterized for heat stress and freezing tolerance. Using comparative genomic hybridizations, we first assessed the genetic variation among ten selected different wild potato accessions. The same species were subjected to heat stress and tissue samples were collected 3, 6 and 9 days after initiation of the heat stress. Distinct expression patterns were found for heat resistant and sensitive accessions. The same potato accessions were used to address freezing tolerance based on their germplasm database descriptors. Potato leaves were subjected to freezing and samples were collected over a temperature range of 0C to -12C. In addition to the transcriptional responses, ion leakage from the leaves was measured to assess freezing damage. All expression data will be made publicly available at www.tigr.org/tdb/potato/.


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1 - The Institute for Genomic Research, 9712 Medical Center Dr, Rockville, MD, 20850, US

Keywords:
abiotic stress
microarary
expression profiling.


Session: PAA05-3
Location: Hall of Ideas Room F/Monona Terrace
Date: Thursday, July 27th, 2006
Time: 9:00 AM
Abstract ID:25


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