| Abstract Detail
Translational Genomics - Morning Darrigues, Audrey [1], Yang, Wencai [2], van der Knaap, Esther [1], Hogenhout, Saskia [3], Francis, David [1]. DNA-Microarray detection of molecular markers for improving color and nutritional quality in tomato. COLOR is among the most important attributes of tomato for processing into whole and diced products. Both color and color uniformity are greatly affected by Yellow Shoulder Disorder (YSD), a ripening disorder that results in discoloration of the proximal end tissues of the fruit. Lycopene and beta-carotene concentrations are reduced by 18% and 22%, respectively, in fruits affected by YSD. To discover markers for use in the genetic analysis of YSD, a custom DNA microarray was hybridized with target cDNA from Ohio 7814 and LA1589. We developed algorithms to detect outliers, and identified 1,296 putative SFPs. Subsequent sequence verification confirmed 52% of the features identified as outliers. In general, residual and confidence interval based approaches had a higher verification rate than difference based approaches. These markers are being mapped and screened for utility on a collection of 92 S. lycopersicum lines We used the tomato analyzer software to measure color and color uniformity (http://www.oardc.ohio-state.edu/vanderknaap/tomato_analyzer.htm) in three inbred backcross populations derived from either S. lycopersicum x S. lycopersicum or S. lycopersicum x S. pimpinellifolium crosses. Marker-trait association analysis from this study reveals QTL from S. lycopersicum that contribute to both color and color uniformity.
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Related Links:
software for the analysis of fruit shape, color, and size
1 - The Ohio State University, Horticulture and Crop Science, 1680 Madison Ave., Wooster, OH, 44691
2 - China Agricultural University, Vegetable Sciences, College of Agronomy and Biotechnology, No.2 Yuanmingyuan Xi Lu, Beijing, 100094, China
3 - The Ohio State University, Entomology, 1680 Madison Ave., Wooster, OH, 44691, USA
Keywords:
microarray
SNP
lycopene
color.
Session: Poster-54
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:240 |
