| Abstract Detail
Secondary Metabolism - Afternoon Rogachev, Ilana [1], Venger, Ilya [1], Mandel, Tali [1], Aharoni, Asaph [1]. Using Ultra Performance Liquid Chromatography (UPLC)-QTOF-MS for Metabolome Analysis of Tomato Fruit Peel. THE peel of fruit acts as a protective barrier and as a medium for the exchange of gases and water. We study the peel of tomato to unravel the network of genes and proteins which determine the unique characteristics of this tissue. Four major metabolic pathways synthesizing cutin, wax, triterpenoids, and aromatic metabolites are active in tomato peel. Our analytical platform includes UPLC coupled to a Q-TOF-MS instrument. The UPLC system detects more peaks with faster run times compared to HPLC. Methanol extracts of tomato flesh and peel tissues derived from four developmental stages were separated with a seven minutes gradient and analyzed by MS in ESI positive and negative modes. For data analysis we used the MarkerLynx program that conducted mass signals alignment across samples and identified differential markers. Gross changes in levels of secondary metabolites were detected through peel development. We also examined a natural tomato mutant termed "y" showing a colorless peel phenotype compared to the yellow-colored normal peel. Peel and flesh tissue derived from four developmental stages of the "y" mutant were profiled. Data interpretation by means of PCA showed that most changes in the "y" mutant occur in the orange/red stages of development. Apart from a decrease in the levels of the yellow flavonoid naringenin chalcone, levels of naringenin and possibly its derivatives were decreased in the "y" mutant. Metabolites with elevated levels in the mutant peel were also detected. We are currently conducting structural elucidation of differential compounds and generating equivalent data at the Transcriptome level.
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1 - Weizmann Institute of Science, Department of Plant Sciences, Rehovot, 76100, Israel
Keywords:
surface
tomato fruit
flavonoids
metabolomics
Metabolome
Mass spectrometry
mutant.
Session: SAT10-5
Location: Hall of Ideas Room F/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 4:00 PM
Abstract ID:236 |
