| Abstract Detail
Plant Protection Miller, Timothy [1], Stewart, Derek [2], Blok, Vivian [1]. Metabolic profiling of the response in susceptible and resistant Solanaceae to plant parasitic nematodes. PLANT parasitic nematodes are responsible for global crop damage estimated at ~$100 billion annually. Of these, the obligate endoparasitic root-knot (textMeloidogynetext) and cyst (textGloboderatext and textHeteroderatext spp.) nematodes are amongst the most important causes of yield losses. These endoparasitic nematodes induce changes in both localized and systemic gene expression leading to either susceptible or resistant interactions with their hosts. Little is known about how these different interactions affect the host’s metabolism and how these relate to changes in gene expression. Metabolite profiling is being used to monitor changes in tomato and potato hosts in compatible and incompatible interactions with textMeloidogynetext and textGloboderatext to determine if these profiles can be used to differentiate these interactions. An extraction protocol has been established for freeze-dried roots and leaves for analysis with gas chromatography mass spectrometry (GC-MS) and high pressure liquid chromatography-photodiode array-mass spectrometry (LC-PDA-MS). This protocol has been tested by comparing metabolite profiles of uninfected tomato with a compatible interaction with textMeloidogyne javanicatext. Significant changes in metabolites have been observed that are characteristic of either compatible or incompatible interactions following profiling of roots from tomato cultivars that are susceptible (Moneymaker) and resistant (Rossol) to textMeloidogyne javanicatext. Results obtained from profiling the leaves of tomato showed changes in metabolites of primary metabolism. Potato genotypes which differ in susceptibility to the potato cyst nematode textGlobodera pallidatext are also being profiled to provide insight into the biochemical basis of susceptible and resistant plant responses and which may provide specific metabolic markers to characterise progeny in potato breeding programs.
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1 - Scottish Crop Research Institute, Plant Pathology Programme, Invergowrie, Dundee, Perthshire, DD2 5DA, Scotland, UK
2 - Scottish Crop Research Institute, Quality, Health and Nutrition Programme, Invergowrie, Dundee, Perthshire, DD2 5DA, Scotland, UK
Keywords:
metabolic profiling
root knot nematode
cyst nematode
Resistance
.
Session: Poster-16
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:234 |
