| Abstract Detail
Conference Wide Di Matteo, Antonio [1], Sacco, Adriana [1], Carputo, Domenico [1], Frusciante, Luigi [1]. Identification of a QTL controlling ascorbate stability in tomato fruit. TOMATO fruits provide a major contribution to human dietary nutrition worldwide, particularly in term of antioxidants. Because antioxidants have been implemented in helping to prevent cancer and heart disease, there is a growing interest in the potential genetic improvement of tomato antioxidant level. Among antioxidants, ascorbate (AsA) play a key role in protecting cells against oxidative damages both in animals and plants. Because of the high instability of AsA content in tomato fruit during storage, the ability of the fruit to better retain AsA over the time is an interesting aspect of the fruit quality. In order to screen genetic variability for QTLs involved in controlling AsA content and stability, we measured AsA in fruits of a Solanum pennellii introgression line (IL) population at red ripe stage and during storage at two different temperatures (4 and 24°C). Such an approach led to the identification of many QTLs affecting AsA content. For example, IL 12-4 showed the higher AsA content. Conversely, IL 9-1 and 9-2 did not showed differential AsA content but performed higher AsA stability. To gain additional insights in the genetic control of AsA stability, we undertook a comparative analysis of IL 9-1 and IL 9-2 by quantitative PCR approach. These lines showed an higher transcription level of a monodehydroascorbate reductase gene (mdhar). This gene was mapped on the short arm of the chromosome 9 of tomato. Mdhar was isolated and sequenced in many Solanaceae. Current efforts aimed at the functional characterization of mdhar by RNA-interference approach are in progress.
Log in to add this item to your schedule
1 - University of Naples Federico II, Department of Soil, Plant and environmental Sciences, Via Università 100, Portici (NA), Italy, 80055
Keywords:
L-ascorbate, monodehydroascorbate reductase, tomato, Solanaceae.
Session: Poster-122
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:231 |
