| Abstract Detail
Breeding and Genetics Rose, Laura [1]. The Age and Diversification of the Pto Resistance Gene Family in the Solanaceae. PHYLOGENETIC analyses were used to determine the age of the Pto gene family and the timing of duplication events of this small gene family relative to speciation events in the Solanaceae. Pto encodes resistance to strains of bacterial speck disease of tomato and orthologs of this gene are found in nearly all species of tomato. In this investigation, a total of 163 orthologs and paralogs from seven species of tomato and one outgroup species, Solanum ochranthum, were cloned and sequenced to investigate the evolutionary origin and history of this gene family. Molecular clock analyses indicate that the origin of the Pto gene coincided approximately with the diversification of the genus Solanum. The specific amino acid changes along the branch subtending the clade of Pto orthologs were inferred and the functional effects of these changes are discussed in the context of what is known about the Pto protein in particular and protein kinases in general. Rates of evolution of the Pto gene family were compared to rates observed at other plant genes to determine if the rate of evolution was faster for an R-gene. A comparison of the rates of evolution among members of the Pto gene family itself did not support the hypothesis that the acquisition or loss of pathogen recognition in particular was correlated with an increased rate of nucleotide substitution in orthologs of Pto.
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1 - University of Munich, Evolutionary Biology, Grosshaderner Str. 2, Planegg, Bavaria, 82152, Germany
Keywords:
adaptation
gene duplication
phylogenetics
tomato
Host plant resistance
coevolution.
Session: PAA07a-3
Location: Hall of Ideas Room G/Monona Terrace
Date: Monday, July 24th, 2006
Time: 2:00 PM
Abstract ID:228 |
