| Abstract Detail
Conference Wide Van Eck, Joyce [1], Tanksley, Steve [2], Giovannoni, Jim [3], Mueller, Lukas [2], Stack, Steve [4]. Sequencing of Tomato Chromosomes 1, 10 and 11: US Contribution to the International Solanaceae Genome Initiative. THE tomato genome is comprised of approximately 950 Mb of DNA with the majority of genes found in long contiguous stretches of gene-dense euchromatin, which accounts for only 25% of the DNA, yet contains more than 90% of the estimated 38,000 genes. As part of an international consortium (ten countries) to sequence the euchromatin of all twelve tomato chromosomes, the US team is sequencing a minimal tiling path of BAC clones through this gene-rich DNA for chromosomes 1, 10, and 11. The remaining nine chromosomes are each being sequenced by an international partner. To date, we have generated three BAC libraries (HindIII, MboI, and EcoRI), obtained BAC end sequence (400,000 reads), and sequenced a sheared library. Each BAC library contains the following number of clones: HindIII, 129,024; MboI, 50,688; EcoRI, 75,000. These libraries, library hybridization filters, and sets of anchor or seed BACs for each chromosome are provided to our sequencing collaborators upon request on a recharge basis with the exception of the seed BACs for which there is no fee. To steer sequencing activities into the euchromatin and away from the heterochromatin, Fluorescence In Situ Hybridization (FISH) is being utilized for BAC localization. We are particularly interested in BACs that are located as close as possible to telomeres and at euchromatin/heterochromatin borders. FISH analysis is also provided as a service to our collaborators for a limited number of BACs. The repository for all information and data generated through this international consortium is the SOL Genomics Network (www.sgn.cornell.edu). A summary of progress to date will be presented.
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Related Links:
SOL Genomics Network
1 - The Boyce Thompson Institute for Plant Research, Cornell University, Tower Rd., Ithaca, NY, 14853, USA
2 - Cornell University, Plant Breeding and Genetics, Tower Rd., Ithaca, NY, 14853, USA
3 - The Boyce Thompson Institute for Plant Research and U.S. Department of, Cornell University, Tower Rd., Ithaca, NY, 14853, USA
4 - Colorado State University, Biology, Fort Collins, CO, 80523-1878, USA
Keywords:
tomato
sequencing
FISH.
Session: Poster-77
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:22 |
