| Abstract Detail
Conference Wide Termolino, Pasquale [1], Cammareri, Maria [1], Massarelli, Immacolata [1], Raimondi, Giampaolo [2], Giovannoni, Jim [3], Maggio, Albino [2], Grandillo, Silvana [1]. Physiological and molecular response to salt stress in Solanum lycopersicum. SALINITY is one of the most severe environmental stresses limiting the productivity of agricultural crops. The cost of salinity to agriculture is very high and is expected to increase as soils are further affected by salinity. Since irrigation is the main cause of soil salinization, salt stress is particularly relevant for horticultural crops, including tomatoes, which are generally irrigated. In this study, the dwarf-like Micro-Tom tomato variety was grown in a closed hydroponic system under two NaCl concentrations (60 mM and 120 mM) that defined a “low” and “moderate” salt stress physiological response. Salinity caused a statistically significant (P < 0,05) decrease in plant fresh weight, leaf area development, leaf number, fresh and dry weights of roots and leaves, whereas shoot/root ratio in salinized plants increased. Total leaf water potential and osmotic potential decreased at increasing salinity of the irrigation water. No significant differences were observed for these traits, between the two NaCl treatments. In order to understand the molecular basis of salt stress response, expression profiles were generated from leaves and roots of plants subjected to 60 mM NaCl, using the 13,440-clone (8700 unigenes) TOM1 microarray. Statistical analyses (t-test P < 0.05) revealed 731 and 676 EST clones differentially expressed in response to salt stress, respectively in leaves and roots. Many genes represented by these clones encoded proteins involved in protein metabolism, signal transduction, primary metabolism and transcriptional regulation. A total of 78 differentially regulated clones were common between leaves and roots. To our knowledge this is the first microarray analysis of tomato response to salt stress and these results will provide new insights into the physiological and molecular basis of salt stress response in tomato.
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1 - Cnr-Igv, Institute of plant genetics, Research Division of Portici, Via Università, 133, Portici, Italy, 80055, Naples
2 - University of Naples Federico II, Department of Soil, Plant and environmental Sciences, Via Università 100, Portici (NA), Italy, 80055
3 - The Boyce Thompson Institute for Plant Research and U.S. Department of, Cornell University, Tower Rd., Ithaca, NY, 14853, USA
Keywords:
Salt stress
tomato
microarray.
Session: Poster-118
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:217 |
