| Abstract Detail
Conference Wide Khurana, Jitendra P. [1]. Progress on Sequencing of Chromosome 5 of Tomato. UNDER the auspices of the International Tomato Sequencing Project, India has committed to sequence about 111 BACs covering the euchromatic region of chromosome 5. This work has been started at three centers in India, i.e. University of Delhi South Campus, National Centre for Plant Genome Research and National Research Centre on Plant Biotechnology. Two tomato BAC libraries (HindIII and MboI) and a set of seed BAC clones for chromosome 5 were obtained in May 2005. A total of 314 seed BAC clones anchored to 45 markers on chromosome 5 have been obtained, replicated and distributed among three centers participating in IITGS. A total of 18 markers were selected as nucleation points for sequencing on both the arms of chromosome 5. Twenty five seed BAC clones assigned to these marker positions were confirmed. Shotgun libraries have been prepared for all of them and high throughput sequencing has started for twenty BAC clones. Out of these three, five and twelve seed BAC clones are at phase III, II and I levels of sequencing, respectively. To extend the tiling path from these nucleation BACs, another set of BAC clones has also been confirmed. The generated sequence contigs were searched in the NCBI database for any homology with the known genes. The BLASTN search was performed at an e-value of 10-20 or less and a cut off bit score of 100. Some important genes such as HAC1 transcription cofactor, UDP-glycosyl transferase, glyceraldehyde-3-phosphate dehydrogenase, nematode resistance-like protein, beta fructosidase gene, polygalactouranase isozyme 1 beta subunit precursor showed their homology to one or the other sequenced BAC clones. Recently, we have also obtained the IL lines of tomato and work is in progress to confirm map position of the BAC clones on tomato chromosome 5. The progress of the work can be followed at http://www.genomeindia.org/tomato/index.html.
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1 - Delhi University South Campus, Plant Molecular Biology, Benito Juarez Road, New Delhi, 110021, India
Keywords:
genome sequencing
tomato.
Session: TBA
Location: /
Date: Thursday, January 1st, 1970
Time: 9:30 AM
Abstract ID:216 |
