| Abstract Detail
Conference Wide McLean, Karen [1], McNicoll, Jane [1], Bradshaw, John [2], Hein, Ingo [1], Milbourne, Dan [3], Bryan, Glenn [2]. Strategies for fine mapping and isolation of resistance QTLs on Linkage Groups IV and V of potato. WE have performed extensive genetic analyses of complex and seemingly durable sources of resistance to the potato cyst nematode (PCN) species Globodera pallida and the late blight pathogen Phytophthora infestans. These investigations, performed at both diploid and tetraploid levels, have identified several QTLs, the largest of which map near resistance hotspots on linkage groups IV and V near the markers STM3016 and GP179 respectively. Map-based cloning of these resistances is rendered difficult by the quantitative and partial nature of the resistances involved and the lack of resolution of QTL maps. An intermediate goal is the development of robust diagnostic molecular markers that can be applied in a modern potato breeding programme. Our approach in attempting to isolate these QTLs relies on use of candidate gene approaches allied to use of information from other potato gene mapping and isolation studies from syntenic regions, as well as tomato genome sequence data. For example, we are attempting to isolate a PCN resistance QTL GpaV introgressed into potato from the wild diploid species S. vernei. We have isolated BAC clones from the resistance locus containing cognate R gene homologues and are currently developing further markers from these BACs as well as from other PCN resistance gene isolation projects. We have developed several new simple sequence repeat markers at this locus. We are adopting a similar strategy for a region on LGIV containing large effect QTLs for resistance to both G. pallida and P. infestans that we suspect is syntenous with the region containing the R2 late blight resistance locus. Markers from other studies are being deployed on an expanded diploid population segregating for the same type of late blight resistance as found in the cultivar Stirling.
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1 - Scottish Crop Research Institute, Genetics Programme, Invergowrie, Dundee, DD2 5DA, UK
2 - Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK
3 - Teagasc Crops Research Centre, Oak Park, Carlow, , Republic of Ireland
Keywords:
Potato
Resistance
PCN
Late Blight.
Session: Poster-116
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:211 |
