| Abstract Detail
Translational Genomics - Morning Granel, Antonio [1], Rambla, Jose Luis [2], Medina, Aurora [3], Alfaro, Cristina [4], Fernandez, Asun [3], Orzaez, Diego [3], Gutierrez, Abelardo [5], Primo, Jaime [4]. A Volatile Phenotyping Platform for Tomato. WE are establishing a volatile phenotyping platform to assess the variability in tomato fruit volatiles present in tomato cultivars, breeding lines, RILs, NILs, and transgenic lines. The platform includes a GC-MS module that is able to identify and quantify up to 68 tomato fruit volatiles, including those normally associated to tomato aroma. Information on and additional over 100 non identified GC-MS peaks is retrieved from each sample. In addition to this volatile profiling platform, a medium-high throughput platform for volatile fingerprinting is being established. To do that tomato fruit samples are homogenized in a 96 well format and subjected later to an electronic nose with 16 sensors to obtain a “noseprint” for each sample in a few minutes. Volatile emission depends on the developmental stage but most are produced upon cell rupture –disorganization: most of the volatiles are produced upon cell disorganization Volatile profiling has been used to assess the level of heterosis in symmetric F1 populations derived from the cross of S. lycopersicon and S. pimpinellifolium. The application of volatile profiling for identifying maternal effects will be discussed. In addition a transient agro-injection and volatile capture and analysis is also used in our group as an extension of the volatile platform to characterize what genes are affecting volatile production in the tomato fruit.
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1 - Consejo Superior de Investigaciones Científicas, Instituto de Biolgia Molecular y Celular de Plantas, Universidad Politecnica de Valencia, Avda Tarongers s/n, Valencia, V alencia, 46022, Spain
2 - Instituto Biolgia Molecular y Celular de Plantas,
3 - Instituto de Biologia Molecular y Celular de Plantas,
4 - Centro Ecologia Quimica Agricola (CEQA),
5 - Valencianos Investigaciones Agrarias, Ce ntro de Agroingeniería, CtraMontcada-Naquera, Km5, Montcada, 46113, Spain
Keywords:
volatiles
tomato.
Session: SAT05-8
Location: Hall of Ideas Room I/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 10:55 AM
Abstract ID:210 |
