| Abstract Detail
Conference Wide Dale, MFB [1], Blok, VC [2], Pylypenko, LA [3], McLean, Karen [1], Bryan, Glenn [2]. SNP markers for evaluating copy number of H1 PCN resistance gene in potato breeding germplasm. THE H1 gene is extremely effective at reducing populations of the potato cyst nematode Globodera rostochiensis throughout the UK and Europe. An important breeding objective is to assess the copy number of the H1 gene in potato breeding lines, permitting clones possessing high dosages of the H1 gene to be identified and employed as breeding parents. Triplex and quadruplex parental material give rise to 100% H1 resistant progeny. The identification of genotypes that are triplex and quadruplex for H1 is difficult and time consuming, requiring extensive resources during the lengthy process of phenotyping derived progenies from crosses between H1-bearing and susceptible parents. We have utilised markers from previous H1 mapping and cloning efforts to develop quantitative single nucleotide polymorphism (SNP) markers flanking the H1 gene that can be used indirectly to measure gene dosage. PCR product sequencing from different H1 resistant and susceptible genotypes and comparison of sequence polymorphism data has been used to develop SNP markers whose dosage can be measured. We have employed a Pyrosequencing method to quantify relative levels of different sequence variants in a DNA sample, and applied this to a range of material with known dosages of the H1 gene. Currently these molecular markers distinguish between presence and absence of the H1 gene, and moreover, can be used to measure H1 gene dosage in some of the material assayed. Results presented demonstrate the potential of this approach for selecting for multiplex resistance status without lengthy backcross programmes and have direct application in potato breeding programmes. This method can be refined as more sequence information at the H1 locus becomes available. The procedures used show promise for application to other major genes common within conventional potato breeding programmes, such as resistance to potato viruses X and Y.
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1 - Scottish Crop Research Institute, Genetics Programme, Invergowrie, Dundee, DD2 5DA, UK
2 - Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK
3 - Institute of Plant Protection UAAS, Plant Quarantine Department, 33, Vasilkovskaya Str., Kiev-22, 03022, Ukraine
Keywords:
PCN
Resistance
nematode
Potato
molecular marker.
Session: Poster-114
Location: Ballroom CD/Monona Terrace
Date: Tuesday, July 25th, 2006
Time: 8:00 AM
Abstract ID:209 |
