| Abstract Detail
Application of FISH in Support of Sequencing Plant Genomes - Afternoon Koo, Dal-Hoe [1], Lee, Sanghyeob [1], Cho, Sung-Hwan [1], Hur, Chul-Goo [1], Park, Hong-Seok [2], Bang, Jae-Wook [3], Choi, Doil [1]. An Integrated Cytogenetic Map of Tomato Chromosome 2. CYTOGENETIC maps incorporate data from molecular linkage maps and chromosomal features such as centromeres, telomeres and, recently, fluorescence in situ hybridization (FISH) signals. We selected 34 molecular markers obtained from RFLP that evenly cover the linkage group of tomato chromosome 2. Tomato chromosome 2 is the NOR-bearing chromosome, and corresponding to tomato linkage group 2. FISH with the bacterial artificial chromosome (BAC) of each of these DNA markers as probes was used to map their position on the meiotic pachytene chromosome. The establishment of this cytogenetic map demonstrated the different ratios of physical/genetic distance between molecular markers. We also observed that the order of the three FISH markers was reversed in relation to their order on the linkage map. Our data indicate that the deduced molecular cytogenetic map will be useful for structural and functional genomics in tomato.
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1 - Korea Research Institute of Bioscience and Biotechnology, Plant Genomics Lab, P.O. Box. 115, Taejon, 305-600, South Korea
2 - Korea Research Institute of Bioscience and Biotechnology, Genomic Research Center, P.O. Box. 115, Taejon, 305-600, South Korea
3 - Chungnam National University, Biology, Chungnam National University, Taejon, 305-764, South Korea
Keywords:
FISH
tomato
cytogenetic map.
Session: SAT06-3
Location: Hall of Ideas Room G/Monona Terrace
Date: Wednesday, July 26th, 2006
Time: 3:15 PM
Abstract ID:207 |
