| Abstract Detail
Breeding and Genetics Iovene, Marina [1], Savarese, Salvatore [1], Cardi, Teodoro [2], Frusciante, Luigi [1], Scotti, Nunzia [2], Simon, Philipp [3], Carputo, Domenico [1]. Assessment of nuclear and cytoplasmic genome composition in Solanum bulbocastanum (+) S. tuberosum somatic hybrids. SOMATIC hybridization via protoplast fusion represents a unique tool to induce genetic variability in both nuclear and cytoplasmic genomes. It also allows the transfer of multiple genes across sexual barriers. Somatic hybridization was performed to introgress useful traits from the wild incongruent species Solanum bulbocastanum (2n=2x=24) into the cultivated potato. ISSR markers led to the identification of 11 S. bulbocastanum (+) S. tuberosum somatic hybrids. Cytological observations revealed the recovering of seven (near) tetraploid and three (near) hexaploid somatic hybrids. Multicolor GISH analysis was carried out in order to study the genomic dosage of the parental species in five somatic hybrids. The procedure employed was effective in discriminating parental genomes in the hybrids since most chromosomes were unambiguously colored. Two tetraploid somatic hybrids showed the expected 2:2 cultivated to wild genomic dosage, two hexaploids revealed a 4:2 cultivated to wild genomic dosage, and one hexaploid had a 2:4 cultivated to wild genomic dosage. Characterization of hybrid cytoplasmic genomes was performed by using gene specific primers that detected polymorphisms between the fusion parents in the intergenic regions. The analysis showed that most of the somatic hybrids inherited the cultivated plastidial and mitochondrial DNA. A few hybrids, with a rearranged mitochondrial genome (showing fragments derived from both parents), were also identified. These results further confirmed the potential of somatic hybridization in producing new variability for genetic studies and breeding purpose.
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1 - University of Naples Federico II, Department of Soil, Plant and environmental Sciences, Via Universitą 100, Portici (NA), Italy, 80055
2 - Cnr-Igv, Institute of plant genetics, Research Division of Portici, Via Universitą, 133, Portici, Italy, 80055, Naples
3 - United States Department of Agriculture-Agricultural Research Service, Vegetable Crops Research Unit, 1575 Linden Dr., Madison, WI, 53706, USA
Keywords:
Somatic hybridization
crossing barriers
GISH
cytoplasmic genomes.
Session: PAA07a-1
Location: Hall of Ideas Room G/Monona Terrace
Date: Monday, July 24th, 2006
Time: 1:30 PM
Abstract ID:202 |
